Bio-AlteR® : Primary culture of seminiferous tubules from pubertal tissue [More details]
Bio-AlteR® Juvenile: Primary culture of seminiferous tubules from juvenile tissue
In vitro toxicology studies of spermatogenesis can be performed from cultures of seminiferous tubules of prepubertal rats (rats 6-8 days, Sprague Dawley, Wistar). Toxicity studies on spermatogenesis are thus performed before the establishment of the blood-testis barrier.
This system is particularly suited to juvenile toxicology application.
Bio-AlteR® Focus: Co-culture of Sertoli cells and purified fractions of germ cell population
This consists in a culture of Sertoli cells in a monolayer, and then addition of different populations of purified germ cells (spermatogonia/preleptotene spermatocytes, pachytene spermatocytes or round spermatids).
This allows making a « zoom » on a specific step of spermatogenesis.
Bio-AlteR® Sertoli Focus: Culture of Sertoli cells , then addition of a total germ cell population
This co-culture allows the treatment of the Sertoli cells independently of the germ cells. Indeed, if only germ cells are altered by the compound, it is likely that spermatogenesis will resume after stopping the treatment, due to the arrival of new waves of differentiating germ cells. Conversely, if Sertoli cells are damaged by the compound, a possible recovery should be less likely, since after the establishment of the blood testis barrier, the population of Sertoli cells is practically fixed for the rest of the life.
Price: We guarantee competitive prices to screen chemicals through our in vitro model. Each quotation fulfills a technical specification approved in advance by the customer.
Confidentiality: All information exchanged with our clients are treated with strict confidentiality.
Delivery time: Timeliness of delivery of our studies is a priority that we manage every day.
Communication: Upon signature of a contract, we schedule a teleconference plan in agreement with our client.
Sertoli cell line (SERW3) : Cytotoxicity tests
Leydig cell line (TM3 ou MA-10): Cytotoxicity tests, androgenic hormones concentration measurements
Primary culture of Leydig cells : Androgenic hormones concentration measurements
Primary culture of Sertoli cells