Advantages

  • Up to 4 weeks time duration of drugs/ chemicals testing
  • Cells grow in a serum free medium
  • Ability to test the substance on differentiating germ cells
  • Drugs/Chemicals  in the basal compartment to mimic the blood compartment
  • Possibility of testing low concentrations of toxic ("physiological")
  • Capacity to evaluate the drugs/chemicals toxic effect reversibility

Contact Us

Kallistem
Ecole Normale Supérieure de Lyon
46 allée d'Italie
69364 Lyon Cedex 07
France

Tel : +33 (0) 4 72 72 89 65
Fax :  +33 (0) 4 26 23 39 50

contact@kallistem.com

Bio-AlteR® 3D Testicular Cell Culture

The solution for reliable, robust and relevant spermatogenesis investigation in vitro

Bio-AlteR® is a novel 3D cell culture of seminiferous tubules enabling spermatogenesis in vitro.

DESCRIPTION
EXPERIMENTAL ENDPOINTS
SPECIES
APPLICATION BY INDUSTRIES

Spermatogenesis in vitro

Bio-AlteR® is an innovative seminiferous tubules 3D cell culture technology enabling the spermatogenesis in vitro. The system mimics physiological conditions. It allows the study of both spermatogenesis mitotic and meiotic phases, as well as the first steps of spermiogenesis. The technology preserves the original 3D structure of the seminiferous tubules, and the Sertoli cells remain polarized which is crucial for the differentiation of germ cells. The blood testis barrier is maintained, which allows the study of drug permeation across the barrier. Thus, this technology bridges the gap between in vitro to the in vivo techniques. 

One well, two compartments
Cells are cultured in a bicameral chamber. This system allows the study of the impact of compounds (substances, drugs or molecules) added into the basal compartment on both the blood testis barrier integrity and on differentiating germ cells. The basal compartment mimics the blood compartment whereas the apical compartment reproduces the seminiferous tubules lumen environment. The cell culture media can be analyzed to identify product release and explore biomarkers produced as a result of toxicity.

Bio-AlteR® Technology by KALLISTEM

Bio-AlteR® has proven its efficacy and reliability for the testing of many molecules such as endocrine disruptors, heavy metals, pesticides, pollutants, others… [Download the list of compounds tested by Bio-AlteR®]

Experimental protocol advantages

  • Up to 4 weeks’ time duration of drugs/ chemicals testing
  • Cells grow in a serum free medium
  • Ability to test the substance on differentiating germ cells
  • Drugs/Chemicals  are added in the basal compartment to mimic the blood compartment
  • Possibility of testing low concentrations of toxicants ("physiological")
  • Capacity to evaluate the drugs/chemicals toxic effect reversibility

Our approach: Testicular "Physio-toxicology"

  • Test the effects of a toxicant on differentiating germ cells
  • Test concentrations of  toxicants  « close to » concentrations found in body fluids (including identified metabolites)
  • Test the effects on relevant  « end points » 
  • In every instance « think of  physiology » 
  • Test the effects of a toxicant at different times of development, if necessary
  • If using  in vitro approaches,  check how these models relate to the  in vivo (physiological) situation
  • In order to prevent "false negative" results experimental models must allow testing  concentrations of  toxicants close to those found in biological fluids (Geoffroy-Siraudin et al., 2010; 2012; Perrard et al., 2013). 
  • Also, in order to prevent "false positive" results, particular attention should be given to the concentrations of toxicants which are experimentally tested, and do not work with “excessive” doses.

Bio-AlteR® Technology by KALLISTEM Modification of cell number population

Bio-AlteR® Technology by KALLISTEM Blood testis barrier integrity  

Bio-AlteR® Technology by KALLISTEM Determination of a meiotic index

Bio-AlteR® Technology by KALLISTEM Analysis of specific cell gene expression

Bio-AlteR® Technology by KALLISTEM Peptide profiling of the culture supernatant/cultured cells

Bio-AlteR® Technology by KALLISTEM


Bio-AlteR® Technology by KALLISTEM

Spermatogenese by KALLISTEM

 

Bio-AlteR® Technology by KALLISTEM

Cross-species comparison studies are possible using Bio-AlteR® to better understand the mechanism(s) involved in a testicular toxic effect.

 

 

Pharmaceuticals Chemicals Agrochemicals

 

 

Cosmetics Environmentals